diff --git a/Jellyfin.Api/Controllers/DynamicHlsController.cs b/Jellyfin.Api/Controllers/DynamicHlsController.cs
index 96b4319f18..40bd26433e 100644
--- a/Jellyfin.Api/Controllers/DynamicHlsController.cs
+++ b/Jellyfin.Api/Controllers/DynamicHlsController.cs
@@ -1424,8 +1424,8 @@ public class DynamicHlsController : BaseJellyfinApiController
         double fps = state.TargetFramerate ?? 0.0f;
         int segmentLength = state.SegmentLength * 1000;
 
-        // If framerate is fractional (i.e. 23.976), we need to slightly adjust segment length
-        if (Math.Abs(fps - Math.Floor(fps + 0.001f)) > 0.001)
+        // If video is transcoded and framerate is fractional (i.e. 23.976), we need to slightly adjust segment length
+        if (!EncodingHelper.IsCopyCodec(state.OutputVideoCodec) && Math.Abs(fps - Math.Floor(fps + 0.001f)) > 0.001)
         {
             double nearestIntFramerate = Math.Ceiling(fps);
             segmentLength = (int)Math.Ceiling(segmentLength * (nearestIntFramerate / fps));